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Home > Company News

ELISA kit 17 related operation tips

Views : 1922
Update time : 2023-02-01 09:30:28
1. Remember to wipe the surface of the enzyme plate lightly with absorbent paper after adding enzyme reagent to absorb dryness.
2. Reasonably arrange the assay volume to avoid too many reaction plates resulting in long waiting time for plate washing.
3. When aspirating the liquid, use a gun with a range close to the required amount to reduce the error.
4. Be sure to do a double-well experiment, so as to ensure both the accuracy of the data and reflect the precision of the kit.
5. Sample dilutions should be refilled with a sparger and proofread frequently for accuracy.
6. To prevent the sample from evaporating, the reaction plate should be placed in a closed box with a wet cloth, and the enzyme plate should be covered or laminated.
7. Store unused plates or reagents at 2-8°C. Horseradish peroxidase-labeled anti-human IgG working solution should be used in the amount required. Do not reuse diluted Horseradish Peroxidase-labeled Anti-Human IgG working solution.
8. In ELISA kit experiments, put the sample into the incubator in time after addition, and operate in batches when there are more specimens. Strictly control the operating time according to the steps in the instructions to prevent the incubation time from being artificially extended, resulting in non-specific binding tightly attached around the reaction wells and difficult to clean thoroughly.
9. The remaining samples and waste should be autoclaved at 121℃ for 30 minutes or treated with disinfectant such as 5.0g/L sodium hypochlorite for 30 minutes and then discarded.
10. Each well of the human ELISA kit should be filled with liquid when washing to prevent free enzymes from being washed out of the pore.
11. Each experiment leave a hole as a blank zeroing hole, the hole does not add any reagent, only the last add substrate solution and 2N H2SO4. measurement first use this hole to adjust the OD value to zero.
12. Wash the plate manually each time after adding the washing solution. Do not spill the washing solution in one enzyme standard solitaire into another enzyme standard well to prevent cross contamination. After shaking off the washing solution, put the enzyme plate on a towel or absorbent paper and pat dry.
13. When in doubt about the results of a sample, it is necessary to use other assays for verification.
14. If deionized water or double-distilled water is not available, use Wahaha pure water to prepare the solution, do not use tap water.
15. In the use of microsampler, aspirate different bottles of liquid to replace the tip, even if aspirate the standard solution.
16. Aspirate the liquid is not easy to too fast, so as not to produce bubbles, the amount of human ELISA kit is not accurate.
17. when aspirating liquid to choose the range and the need to be close to the amount of microvolume spiker suction, to reduce the error.
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