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Yeasen Luciferase Reporter Gene Assay Kits，The signal half-life is up to 5 hours，Worry-free High-throughput Drug Screening

Yeasen Luciferase Reporter Gene Assay Kits，The signal half-life is up to 5 hours，Worry-free High-throughput Drug Screening

Mar 26, 2026

03-262026

Luciferase reporter gene Assay Kits, as an indispensable core tool in drug development, play a crucial role in key processes such as high-throughput screening, efficacy evaluation, and target validation due to their ability to monitor gene expression and signal transduction in real-time. The application of this technology has significantly improved the efficiency of drug screening and evaluation, accelerating the discovery and development of new drugs.
How to Choose the Right Secondary Antibody

How to Choose the Right Secondary Antibody

Mar 01, 2026

03-012026

A secondary antibody is an antibody that can bind to a primary antibody. It is mainly used to detect the presence of the primary antibody. The primary antibody specifically binds to the antigen, and the secondary antibody specifically binds to the primary antibody. In other words, the Fc region or Fab fragment of the primary antibody can act as an antigen to stimulate the body to produce antibodies. That is to say, the primary antibody is mainly IgG class immunoglobulins, so the secondary antibody is an anti-IgG antibody.
Guide to Choosing Loading Control and Epitope Tagging Antibodies

Guide to Choosing Loading Control and Epitope Tagging Antibodies

Feb 03, 2026

02-032026

Dear friends, do you feel at a loss when facing various Loading Control and Epitope Tagging Antibodies during protein immunoblotting? Loading Control proteins are encoded by housekeeping genes in organisms and are stably expressed in multiple tissues.
ELISA kit spiking problems and solutions

ELISA kit spiking problems and solutions

Mar 01, 2023

03-012023

In ELISA experimental operation, sample addition is an important step, and this step has a great influence in the whole experiment. To organize this issue.
Features and principles of Elisa kits

Features and principles of Elisa kits

Mar 01, 2023

03-012023

sensitive and specific antibodies. high reproducibility and reliability.good adsorption performance, low blank value, solid phase carrier with high transparency at the bottom of the pore.suitable for serum, plasma, tissue homogenate, cell culture supernatant, urine and many other specimen types.Complete range of assay indicators: cytokine assay, myocardial infarction assay, endocrine assay, liver fibrosis assay, metabolic and other frightened animals
ELISA experiment steps and precautions

ELISA experiment steps and precautions

Mar 01, 2023

03-012023

I. Encapsulated antigen

Dissolve the antigen with 50 mM carbonate coating buffer (pH 9.6) to a concentration of 10-20 μg/ml, add 100 μl/well to a 96-well enzyme plate, and leave it overnight at 4℃.After discarding the coating solution the next day, wash the plate 3 times with PBST and add 150 μl of 1% BSA to each well for 1 hour at 37℃.
Notes on the double antibody sandwich method

Notes on the double antibody sandwich method

Mar 01, 2023

03-012023

Soluble antigen or antibody is adsorbed on the solid phase carrier and becomes insoluble form, which is the basic condition for enzyme labeling assay. Many substances can be used as solid-phase carriers, such as cellulose, cross-linked dextrose, agarose beads, polypropylene, polystyrene, polyethylene and polyvinyl chloride, etc..
Introduction of the double antibody sandwich method

Introduction of the double antibody sandwich method

Mar 01, 2023

03-012023

The double antibody sandwich method is suitable for the determination of large molecule antigens that are bivalent or above, but not for the determination of semi-antigens and small molecule monovalent antigens, as they cannot form a two-dot sandwich. Today I will talk to you about why the double antibody sandwich method is a common method for antigen detection?
Selection and optimization of antibody dilution of Abcam

Selection and optimization of antibody dilution of Abcam

Feb 28, 2023

02-282023

The binding rate between antibody and antigen depends on the afinity constant. The affinity constant can be affected by temperature, pHl and bufer composition. The formation of antibody antigen complexes can also be controlled by changing the relative concentration of antibody and antigen in solution. Since tis usualy impossible to change the concentration of the antigen. the optimal working concentration (dilution) of each antiboy must be determined for each application and each experimental condition
ELISA kit 17 related operation tips

ELISA kit 17 related operation tips

Feb 01, 2023

02-012023

Remember to wipe the surface of the enzyme plate lightly with absorbent paper after adding enzyme reagent to absorb dryness.Reasonably arrange the assay volume to avoid too many reaction plates resulting in long waiting time for plate washing.
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