Today I will introduce you to the features and principles of Elisa kits.
Features.
1, sensitive and specific antibodies.
2. high reproducibility and reliability.
3, good adsorption performance, low blank value, solid phase carrier with high transparency at the bottom of the pore.
4, suitable for serum, plasma, tissue homogenate, cell culture supernatant, urine and many other specimen types.
5. Complete range of assay indicators: cytokine assay, myocardial infarction assay, endocrine assay, liver fibrosis assay, metabolic and other frightened animals
6. Quality assurance and big savings on experimental expenses.
Principle.
ELISA is based on the solid phase of an antigen or antibody and the enzymatic labeling of the antigen or antibody. The antigen or antibody bound to the surface of the solid phase carrier retains its immunological activity, and the enzyme-labeled antigen or antibody retains both its immunological and enzymatic activity. During the assay, the specimen under test (in which the antibody or antigen is measured) reacts with the antigen or antibody on the surface of the solid phase carrier. The antigen-antibody complex formed on the solid-phase carrier is separated from the rest of the liquid by washing. Then the enzyme-labeled antigen or antibody is added and also bound to the solid phase carrier by the reaction. At this point, the amount of enzyme on the solid phase is in proportion to the amount of the substance under test in the specimen. After adding the substrate of the enzyme reaction, the substrate is catalyzed by the enzyme to become a colored product, and the amount of the product is directly related to the amount of the tested substance in the specimen, so it can be analyzed qualitatively or quantitatively according to the shade of the color presentation.
Note the storage of Elisa kit: 2-8℃ (for frequent use); -20℃ (for longer time without use).